VUMC Functional Genomics Core

Overview of Services

The FGI Core will provide pooled CRISPR screening and primary human hematopoietic cells.  Forward genetic screens have been foundational in lower model systems but challenging in primary human or mouse cells.  CRISPR technology now allows highly efficient pooled forward genetic screening.  The Functional Genomics & Immunology core is based on technology developed in the Rathmell lab and collections of deidentified human peripheral blood by the Vanderbilt Center for Immunobiology.  The core will generate and manage pooled gRNA screening libraries for CRISPR and perform screening assays and analyses.  The core will also collect and distribute deidentified primary healthy donor peripheral blood samples from leukapheresis.  These resources will provide essential immunological research material from clinical patients and translational animal models; to facilitate the application of functional genomics pooled CRISPR screening to identify gene targets; and promote collaboration among research groups with different interests and expertise.

Leadership

Jeff Rathmell, Ph.D., Core Scientific Director

Location and hours of operation

Links and Resources

VUMC Functional Genomics Core website: https://www.vumc.org/functionalgenomicscore

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