IA-VUMC Rat Metabolic Physiology Core (RMPC)

Overview of Services

The Metabolic Physiology Shared Resource (MPSR) (Kevin Niswender, MD, Ph.D., Director) assists investigators wishing to address hypotheses related to diabetes and metabolism. The Rat Metabolic Physiology Core (RMPC) is a section of the MPSR that provides novel techniques to better understand specifically rat models of diabetes and its complications.

The RMPC offers a variety of services including:

Chronic artery &/or vein cannulation
Chronic ileal vein cannulation
Chronic portal vein cannulation
Insulin clamps
Pancreatic clamps
and more...

Our Goals

broaden the scope of techniques available to investigators
standardize key methodologies
expedite the completion of research

*The Vanderbilt University Medical Center (VUMC) Metabolic Physiology Shared Resource Core (MPSR) is supported by Diabetes Research Training Center (DRTC) NIH grant DK020593. Please acknowledge this in your publications*

Leadership

Kevin Niswender, MD, PhD, Core Director

Tiffany Farmer, MS, Lab Manager

Alisa Escue, Core Administrator

Location and hours of operation

Hours	Location
8 am - 4 pm, Monday - Friday	807 LH

Links and Resources

https://www.vumc.org/mpsr

Services & Rates

Name	Description	Price
Carotid Artery + Jugular Vein	Double catheter Typically used for: Insulin clamp, Hyperglycemic clamp, DMPK, Tolerance test	Inquire
Carotid Artery + Jugular Vein + Ileal Vein	Triple catheter Typically used for: Pancreatic clamp, Insulin clamp	Inquire
Carotid Artery + Jugular Vein + Ileal Vein + Portal Vein	4 catheter Typically used for pancreatic clamp	Inquire
Charles River - Rat	Each Pricing is determined by Charles River catalog based on age/size, species, and gender	Inquire
Cisterna Magna	Chronic single catheter placed in cisterna magna for CSF sampling over time	Inquire
CLAMS Energy Expenditure (per rat/day)	Comprehensive Laboratory Animal Monitoring System (CLAMS) Charged per rat per day Energy Expenditure, price includes 5008 meal. Alternative diet charged separately.	Inquire
Glucose Tolerance Test (GTT)	Blood sampled via arterial catheter Oral, Intravenous, or Intraperitoneal * Price does not include cost of surgery, injection, or personnel time.	Inquire
Insulin Clamp	Blood sampled via arterial catheter Peripheral infusion of Insulin Peripheral infusion of Glucose to maintain desired concentration (Optional) Peripheral infusion of 3-3H-Glucose to measure endogenous glucose production (Optional) Bolus of 14C-2-Deoxyglucose to measure muscle glucose uptake *Price of surgery not included	Inquire
Insulin Tolerance Test (ITT)	Blood sampled via arterial catheter Oral, Intravenous, or Intraperitoneal * Price includes supplies. Does not include cost of surgery, injection, or personnel time.	Inquire
Osmotic Pump &/or Pellet	Subcutaneous implant Pump is to be prepared by investigator	Inquire
Pancreatic Clamp	Blood sampled via arterial &/or portal vein catheter Peripheral infusion of somatostation to inhibit endogenous production of insulin & glucagon Intraportal infusion of insulin and glucagon at set concentrations Peripheral infusion of glucose to maintain desired concentration (Optional) Peripheral infusion of 3-3H-Glucose to measure endogenous glucose production (Optional) Bolus of 14C2DG to measure muscle glucose uptake *Price of surgery not included	Inquire
Personnel (per day)	Per day	Inquire
Personnel (per hour)	Per hour	Inquire
Rat DMPK Study	Blood sampled from arterial catheter Administration of substrate(s) by single infusion or bolus via venous catheter, oral gavage, IP injection, or SC injection * Price does not include cost of surgery, injection, or extra personnel time.	Inquire
Single Vessel Catheter	Carotid artery, Femoral artery, Jugular vein, or Femoral vein	Inquire
Tissue: 14C-2-DeOxyglucose	Per rat	Inquire

Search available services: View: by category alphabetically

Name	Description	Price
▼ ► Procedures - Surgery (6)
Single Vessel Catheter	Carotid artery, Femoral artery, Jugular vein, or Femoral vein	Inquire
Carotid Artery + Jugular Vein	Double catheter Typically used for: Insulin clamp, Hyperglycemic clamp, DMPK, Tolerance test	Inquire
Carotid Artery + Jugular Vein + Ileal Vein	Triple catheter Typically used for: Pancreatic clamp, Insulin clamp	Inquire
Carotid Artery + Jugular Vein + Ileal Vein + Portal Vein	4 catheter Typically used for pancreatic clamp	Inquire
Osmotic Pump &/or Pellet	Subcutaneous implant Pump is to be prepared by investigator	Inquire
Cisterna Magna	Chronic single catheter placed in cisterna magna for CSF sampling over time	Inquire
▼ ► Procedures - Study (6)
Name	Description	Price
Insulin Clamp	Blood sampled via arterial catheter Peripheral infusion of Insulin Peripheral infusion of Glucose to maintain desired concentration (Optional) Peripheral infusion of 3-3H-Glucose to measure endogenous glucose production (Optional) Bolus of 14C-2-Deoxyglucose to measure muscle glucose uptake *Price of surgery not included	Inquire
Pancreatic Clamp	Blood sampled via arterial &/or portal vein catheter Peripheral infusion of somatostation to inhibit endogenous production of insulin & glucagon Intraportal infusion of insulin and glucagon at set concentrations Peripheral infusion of glucose to maintain desired concentration (Optional) Peripheral infusion of 3-3H-Glucose to measure endogenous glucose production (Optional) Bolus of 14C2DG to measure muscle glucose uptake *Price of surgery not included	Inquire
Rat DMPK Study	Blood sampled from arterial catheter Administration of substrate(s) by single infusion or bolus via venous catheter, oral gavage, IP injection, or SC injection * Price does not include cost of surgery, injection, or extra personnel time.	Inquire
Glucose Tolerance Test (GTT)	Blood sampled via arterial catheter Oral, Intravenous, or Intraperitoneal * Price does not include cost of surgery, injection, or personnel time.	Inquire
Insulin Tolerance Test (ITT)	Blood sampled via arterial catheter Oral, Intravenous, or Intraperitoneal * Price includes supplies. Does not include cost of surgery, injection, or personnel time.	Inquire
CLAMS Energy Expenditure (per rat/day)	Comprehensive Laboratory Animal Monitoring System (CLAMS) Charged per rat per day Energy Expenditure, price includes 5008 meal. Alternative diet charged separately.	Inquire
▼ ► Personnel Time (2)
Name	Description	Price
Personnel (per day)	Per day	Inquire
Personnel (per hour)	Per hour	Inquire
▼ ► Analysis (1)
Name	Description	Price
Tissue: 14C-2-DeOxyglucose	Per rat	Inquire
▼ ► Supplies - Animals (1)
Name	Description	Price
Charles River - Rat	Each Pricing is determined by Charles River catalog based on age/size, species, and gender	Inquire