Overview of Services
- Mouse tissue collection: Aortas are flushed (30 ml PBS) through the left ventricle, the heart with ascending aorta is isolated, embedded in OCT and snap-frozen in liquid nitrogen. Different tissues including aortas are dissected and collected.
- Analysis of atherosclerotic lesions in the proximal aorta: Cryosections of 10-µm thickness are taken from the proximal aorta starting from the end of the aortic sinus ) and stained with Oil-Red-O with hematoxylin. The image analysis is performed using the KS 300 system.
- Analysis en face of aortic atherosclerotic lesions: The aortas are dissected, pinned out for en face and stain with Sudan IV. Morphometry of lesions is performed via image capture and computer analysis of lesion area.
- Cryosectioning of Tissue Samples.
- Plasma Lipids: Total plasma cholesterol and triglyceride are measured by standard enzymatic assays using a microtiter plate reader (Raichem Co).
- Lipoprotein Fractionation and Characterization: Lipoprotein fractions (chylomicrons, VLDL, LDL, HDL) are isolated by fast protein liquid chromatography (FPLC).
- Consultation and Education: The Atherosclerosis Subcore provides consultation and advice regarding the experimental design and appropriate utilization of the methods for analysis of atherosclerosis.
Leadership
Scientific Director: MacRae Linton, M.D.
For general questions or assistance: john.l.blakemore@vumc.org or youmin.zhang@vumc.org
Location and hours of operation
Hours |
Location |
Monday - Friday 8 A.M. - 5 P.M.
|
312 PRB |
Links and Resources
- https://labnodes.vanderbilt.edu/resource/view/id/10820/community_id/1418